Human-caused dramatical increase in gaseous nitrogen (N) emissions to the atmosphere has accelerated terrestrial ecosystem N deposition over the past half century. As forest productivity is usually N limited, the accelerated N deposition can promote the growth of forest trees. But long-term excessive N input may negatively affect forest ecosystems, leading to soil acidification nutrient loss, plant nutrient imbalance, greenhouse gas emissions increase and biodiversity loss.

Nitrification is the process by which microbes convert ammonium-N or soluble organic N to nitrate-N. Both ammonium- and nitrate-N can be directly used by plants and microorganisms.  But nitrate-N is prone to be leached to lower reaches of waterbodies, thereby causing loss of soil nutrients and eutrophication. Nitrate-N is the substrate of denitrification (the process of NO₃-N being converted to N₂). Higher amount of Nitrate-N enhances denitrification. Nitrification and denitrification are important microbial processes influencing the greenhouse gas N₂O emissions, N availability and composition, and the ecosystem N cycling. Quantifying soil nitrification rate on catchment scale is a necessary step to quantify denitrification. However, due to the spatial and temporal heterogeneity of forest soil nitrification, it has been a difficulty in quantifying ecosystem-scale nitrification rate.

FANG Yunting and others (2015, PNAS) for the first time determined the annual nitrification rate of a forest ecosystem by quantifying the oxygen isotope Δ¹⁷O of nitrate-N in rainfall and streams. However, because the quantification must rely on the measurement of the nitrate-Δ¹⁷O, only a few laboratories in the world have test capabilities. Therefore, there is still little such research and it is currently unknown about the seasonal and inter-annual changes in forest soil nitrification and the drivers controlled these changes.

In view of this, the researchers (HUANG Shaonan, FANG Yunting et al.) from the Stable Isotope Ecology Group of the Institute of Applied Ecology (IAE), Chinese Academy of Sciences, collected rainfall and stream water samples from a 536 ha forest catchment near to the CAS Qingyuan Forest Ecosystem Research Station from 2014-2017.

They measured Δ¹⁷O of nitrate-N in the samples and quantified the nitrate-N input, loss and ecosystem-scale gross nitrification rate. The results showed that the nitrate-Δ¹⁷O in rainfall was greatly diluted when it enters the soil. The soil nitrification showed wide inter-annual variations. But the study didn't find that there was a clear relation between the monthly dynamics of soil nitrification and precipitation or soil temperature. The amount of loss of nitrate-N was higher than the critical value (5 kg N ha^-1 yr^-1) marking the N-saturation of temperate forests. The loss of gaseous N accounted for 35% of total N loss, while the total N output was 56% of the total N input from bulk precipitation.

The researchers thus preliminarily confirmed that the forest ecosystem have been N-saturated. The study on catchment-scale nitrification will be beneficial to understand N cycling and N status of forest ecosystems.

The study entitled “Multiyear measurements on Δ¹⁷O of stream nitrate indicate high nitrate production in a temperate forest” has been published in Environmental Science & Technology.

This study was funded by the National Natural Science Foundation of China, the National Key Research and Development Program, the CAS Key Scientific Research Project of the Frontier Sciences, and the CAS international research team project.

Email: yueqian@iae.ac.cn

Publication Name: HUANG Shaonan et al.